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American Society of Microbiology ABMM Exam Syllabus Topics:
| Section | Objectives |
|---|---|
| Topic 1: Clinical Microbiology | - Bacteriology
|
| Topic 2: Laboratory Management & Quality Systems | - Quality assurance and quality control
|
| Topic 3: Infectious Disease Principles | - Epidemiology
|
| Topic 4: Laboratory Operations | - Diagnostic workflow
|
American Society of Microbiology ABMM Sample Questions:
1. A Gram stain of cerebrospinal fluid (CSF) from a 2-year-old child with meningitis shows small, pleomorphic Gram-negative coccobacilli. Culture on chocolate agar yields growth, but there is no growth on standard 5% sheep blood agar unless streaked near a colony of Staphylococcus aureus. This phenomenon indicates a requirement for which growth factor(s)?
A) Factor X (hemin) only
B) Factor V (NAD) only
C) Cysteine and pyridoxal
D) Both Factor X and Factor V
2. A clinical microbiology laboratory is investigating an outbreak of vancomycin-resistantEnterococcus faecium (VRE) in a hospital. Whole-genome sequencing of isolates from different patients reveals the presence of the vanA operon on a mobile genetic element that is highly conserved across all isolates. This finding MOST strongly suggests:
A) Selection of vancomycin-resistant mutants within a susceptible E. faecium population due to antibiotic pressure.
B) Clonal spread of a single VRE strain carrying the vanA operon.
C) Independent acquisition of vancomycin resistance by different E. faecium strains.
D) Horizontal transfer of the vanA operon to multiple susceptible E. faecium strains.
3. A clinical microbiology laboratory is validating a new point-of-care test for the rapid detection of influenza A and B viruses. To accurately determine the clinical sensitivity of the test, it should be compared to a gold standard method using:
A) A large number of prospectively collected clinical samples from patients with suspected influenza, with results confirmed by RT-PCR.
B) Spiked samples containing known concentrations of influenza antigens.
C) A panel of known positive and negative samples stored at room temperature.
D) Samples from patients with other respiratory viral infections to assess cross-reactivity.
4. A microbiology laboratory isolates a Gram-negative rod from a wound infection. The organism is oxidase- negative, ferments lactose on MacConkey agar, and produces indole. The MOST likely identification is:
A) Proteus mirabilis
B) Klebsiella pneumoniae
C) Escherichia coli
D) Serratia marcescens
5. For epidemiological tracking and outbreak investigations of Clostridioides difficile infection (CDI), which molecular typing method based on PCR amplification targeting repetitive elements within the pathogenicity locus (PaLoc) has historically been widely used and designated by specific nomenclature (e.g., NAP1/BI
/027)?
A) Multilocus sequence typing (MLST)
B) PCR Ribotyping
C) Pulsed-field gel electrophoresis (PFGE)
D) Whole-genome sequencing (WGS) SNP analysis
Solutions:
| Question # 1 Answer: B | Question # 2 Answer: B | Question # 3 Answer: A | Question # 4 Answer: C | Question # 5 Answer: B |
